Re: Quantifying Stained Retina

Posted by NatyC on
URL: http://imagej.273.s1.nabble.com/Quantifying-Stained-Retina-tp5000880p5000993.html

On 11/28/12, Natalia Chacon <[hidden email]> wrote:

> Dear Mr. Gabriel,
>
> I've enclosed another image that seems to contain more iron in the sample.
> Also, is it still possible to use the Color Deconvolution to get the
> data my mentor desires?
>
> Thanks,
> Natalia
>
> On 11/20/12, Gabriel Landini <[hidden email]> wrote:
>> On Tuesday 20 Nov 2012 14:55:39 Natalia Chacon wrote:
>>> Rob, my mentor told me she used a stain called "Pearls Blue"
>>>
>>> Joel, I'm interested in quantifiying most of the stainings visible.
>>> (the background staining I don't desire to quantify.)
>>>
>>> What you're stating though does correlate with what my mentor has
>>> informed
>>> me. That the onl nuclei would be the most dense.  This would be due to
>>> the
>>> way the protien settles.
>>
>> This is what Prussian, Pearls or Berlin blue looks like:
>>
>> http://www.jichi.ac.jp/pathology/swfu/d/fe-10.jpeg
>>
>> Your image looks like all contrast stain to me. So you might not have any
>> iron
>> compounds there or it is so faint that the contrast stain prevails.
>>
>> With Berlin blue, you can *demonstrate* that some iron compound is there,
>> but
>> I am not sure if it can be used to quantify their quantity. Here it says
>> it
>>
>> cannot, so I would be careful how to interpret the intensity:
>>
>> http://stainsfile.info/StainsFile/stain/pigment/perls.htm
>>
>> Regards
>>
>> Gabriel
>>
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>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>>
>

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