http://imagej.273.s1.nabble.com/Quantifying-Stained-Retina-tp5000880p5001009.html
Measurement on a Split Channel (RGB). With this process I measured the
> Hi,
> this is an interesting thread with many very differing layers of
> understanding of images!
>
> What about measuring the "amount" of blue, which is IMHU the total
> extinction of the blue channel, in a selected region, e.g. a layer or a part
> of it? For standardization the area of the region should be measured too, or
> if the nuclei are of interest, better the area of the nuclei inside the
> selected region.
>
> ... and so on, step by step a real measurement scheme will be established.
>
> For reliable measurements lots of questions will arise! If "the iron"
> stained by "Pearls Blue" is in any sense stoichiometric perhaps images
> should be sampled as pure intensity image using the appropriate filter!
>
> However, I would recommend to measure, as the mentor ask for, and look at
> the results.
> Also mentors are still learning!
>
> Regards
> Karsten
>
> Am 28.11.2012 um 21:55 schrieb Natalia Chacon:
>
>> On 11/28/12, Natalia Chacon <
[hidden email]> wrote:
>>> Dear Mr. Gabriel,
>>>
>>> I've enclosed another image that seems to contain more iron in the
>>> sample.
>>> Also, is it still possible to use the Color Deconvolution to get the
>>> data my mentor desires?
>>>
>>> Thanks,
>>> Natalia
>>>
>>> On 11/20/12, Gabriel Landini <
[hidden email]> wrote:
>>>> On Tuesday 20 Nov 2012 14:55:39 Natalia Chacon wrote:
>>>>> Rob, my mentor told me she used a stain called "Pearls Blue"
>>>>>
>>>>> Joel, I'm interested in quantifiying most of the stainings visible.
>>>>> (the background staining I don't desire to quantify.)
>>>>>
>>>>> What you're stating though does correlate with what my mentor has
>>>>> informed
>>>>> me. That the onl nuclei would be the most dense. This would be due to
>>>>> the
>>>>> way the protien settles.
>>>>
>>>> This is what Prussian, Pearls or Berlin blue looks like:
>>>>
>>>>
http://www.jichi.ac.jp/pathology/swfu/d/fe-10.jpeg>>>>
>>>> Your image looks like all contrast stain to me. So you might not have
>>>> any
>>>> iron
>>>> compounds there or it is so faint that the contrast stain prevails.
>>>>
>>>> With Berlin blue, you can *demonstrate* that some iron compound is
>>>> there,
>>>> but
>>>> I am not sure if it can be used to quantify their quantity. Here it
>>>> says
>>>> it
>>>>
>>>> cannot, so I would be careful how to interpret the intensity:
>>>>
>>>>
http://stainsfile.info/StainsFile/stain/pigment/perls.htm>>>>
>>>> Regards
>>>>
>>>> Gabriel
>>>>
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>>>> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>>>>
>>>
>>
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