Re: counting cells

Posted by dscho on
URL: http://imagej.273.s1.nabble.com/counting-cells-tp5001091p5001102.html

Hi Thuraya,

On Mon, 10 Dec 2012, Thuraya Medhat wrote:

> i need help how to count cells with the dark purple color that exist in the
> big circle area

It looks like you want to use the Colour Deconvolution plugin of Gabriel
Landini:

        http://www.dentistry.bham.ac.uk/landinig/software/software.html

Then you will need the Particle Analyzer:

        http://mirror.imagej.net/docs/guide/146-30.html#toc-Subsection-30.2

> please help me if you can

Sure, that is what this mailing list is for. But you will find that your
studies will require a good deal of knowledge about what you are doing.

> here is the link for the image,
> http://imageshack.us/photo/my-images/834/46609221.jpg/

In favor of my point above, your images are color images and if you do not
calibrate your camera correctly, the deconvolution results will be ever so
slightly off.

So there is no way around studying a lot about scientific image
processing, I am afraid.

Ciao,
Johannes

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