object-oriented segmentation

Posted by Michiel van Dommelen on Dec 11, 2012; 8:45am
URL: http://imagej.273.s1.nabble.com/object-oriented-segmentation-tp5001110.html

Hi,

Does anyone know a plugin or a method to perform object-oriented segmentation in ImageJ? I have 2 images of the same sample. Image 1 contains a nuclear staining, while image 2 contains a cytoplasmic staining of the same cells. I would like to segment image 2 in such a way that every ROI contains one nucleus from image 1.

What I was trying to do: Segment image 1, make ROIs, transfer ROIs to image 2, and expand ROIs until they either touch another ROI or until they touch the edge of cytoplasmic staining. But the expanding part does not stop when touching another ROI or the edge of cytoplasmic staining.

Another way could be some sort of ROI seeded watershed, but I have no idea to accomplish this.

Can anyone please help me out?

Thanks,
Michiel