Posted by
Krs5 on
Dec 11, 2012; 9:56am
URL: http://imagej.273.s1.nabble.com/object-oriented-segmentation-tp5001110p5001112.html
Dear Michiel,
You could try:
Take your segmented image and process > Binary > Voronoi. Next take the resulting image and Threshold this image with settings that show all the lines. Run Analyze > Analyze Particles with only Add to Manager selected.
Or
Take your original image, Process > Find Maxima and select as Output type: Segmented Particles. Again take the results image and run Analyze > Analyze Particles with only Add to Manager selected.
Hope this works. Best wishes.
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Centre for Core Biotechnology Services
University of Leicester
http://www.le.ac.uk/biochem/microscopy/home.html-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Michiel van Dommelen
Sent: 11 December 2012 08:46
To:
[hidden email]
Subject: object-oriented segmentation
Hi,
Does anyone know a plugin or a method to perform object-oriented segmentation in ImageJ? I have 2 images of the same sample. Image 1 contains a nuclear staining, while image 2 contains a cytoplasmic staining of the same cells. I would like to segment image 2 in such a way that every ROI contains one nucleus from image 1.
What I was trying to do: Segment image 1, make ROIs, transfer ROIs to image 2, and expand ROIs until they either touch another ROI or until they touch the edge of cytoplasmic staining. But the expanding part does not stop when touching another ROI or the edge of cytoplasmic staining.
Another way could be some sort of ROI seeded watershed, but I have no idea to accomplish this.
Can anyone please help me out?
Thanks,
Michiel
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