Selecting round cells as ROIs
Posted by
Burcu on
URL: http://imagej.273.s1.nabble.com/Selecting-round-cells-as-ROIs-tp5001135.html
Hello, I am looking for a way to automatically select (non-adherent, round) T cells as ROIs within an image. My intention is to replicate those regions of interests in the adjacent channels and for instance measure fluorescence intensity. I found a plugin online called 'multi cell outliner' but i cannot get the plugin to work in ImageJ (compilation error). Does anyone have an idea? As the cells are quite round I think a particle identifier could also work.
Here is an example of one of my images(only WF channel):
https://dl.dropbox.com/u/11562565/S3%20C1%2020x%20before%20aCD3%20moving.jpgThanks in advance!
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