Re: object-oriented segmentation
Posted by
Krs5 on
Dec 12, 2012; 6:33pm
URL: http://imagej.273.s1.nabble.com/object-oriented-segmentation-tp5001110p5001140.html
Hi Michiel,
Yes that is the result of using the nuclear image to segment the cytoplasmic signal. Not sure 1,2,3 how to improve that. You could try the watershed plugin from Daniel Saga (
http://bigwww.epfl.ch/sage/soft/watershed/) or Christopher Mei (
http://rsbweb.nih.gov/ij/plugins/watershed.html) to see if they give a better result for this.
Best wishes
Kees
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Michiel van Dommelen
Sent: 12 December 2012 14:03
To:
[hidden email]
Subject: Re: object-oriented segmentation
Hey Kees,
Wauw! Bedankt! This is pretty amazing! Thanks!
There is one thing that worries me a bit. I don’t know if this issue can be solved. In the attached image, you will find “Cytoplasm_Raw-1.jpg” in which the segmentation of the cells is visible. In red I have located some of the issues I am talking about. In these cases, a part of the cells is cut off, which results in an underestimation of cell area. Normally it would not be a big problem, but some of my cell treatments result in a shape change of these cells from “more round” to “more elongated”. And especially the elongated cells will be cut off more easily… Would you see a possible solution for this?
Thanks,
Michiel
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http://imagej.1557.n6.nabble.com/file/n5001136/Cytoplasm_Raw-1.jpg>
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