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Re: I need to copy and crop mutiple ROI from each image

Posted by Pariksheet Nanda on Dec 13, 2012; 1:36pm
URL: http://imagej.273.s1.nabble.com/I-need-to-copy-and-crop-mutiple-ROI-from-each-image-tp5000475p5001142.html

On Sunday, December 9, 2012, Jasvinder S Ahuja wrote:
>
> 1) I cannot save the ROI as an image (.tiff file) On average I have 5-10
> ROI
> per image.


Add your ROIs to the ROI manager. There are two ways to save the ROI
information:
- save to .roi file from the manager
http://rsbweb.nih.gov/ij/docs/menus/analyze.html#manager
- if you just save the .tiff image it should include the ROI drawn on the
image as well, so you will see the ROI the next time you open it in ImageJ.



> I want to save each ROI as an image in a format easy for ImageJ
> to handle.
>

Using the .roi file explained above will accomplish this.


2) Also I am having problems opening deconvolved image files from
> deltavision (*R3D_D3D.dv). Deltavision_opener plugin opens the non
> deconvolved file (*R3D.dv). I believe this should be a simple fix but have
> no clue, on how to go about it.
>

 I don't see support for that file with the LOI Bioformats plugin[1] so
another approach could be to import the raw DeltaVision data into ImageJ
and run deconvolution in ImageJ instead, or have DeltaVision export to a
format ImageJ understands.


Jasvinder
>

 Pariksheet

[1] http://loci.wisc.edu/bio-formats/formats

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