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Re: I need to copy and crop mutiple ROI from each image

Posted by Melissa Linkert-2 on Dec 14, 2012; 2:38am
URL: http://imagej.273.s1.nabble.com/I-need-to-copy-and-crop-mutiple-ROI-from-each-image-tp5000475p5001151.html

Hi,

> 2) Also I am having problems opening deconvolved image files from
> > deltavision (*R3D_D3D.dv). Deltavision_opener plugin opens the non
> > deconvolved file (*R3D.dv). I believe this should be a simple fix but have
> > no clue, on how to go about it.
> >
>
>  I don't see support for that file with the LOI Bioformats plugin[1] so
> another approach could be to import the raw DeltaVision data into ImageJ
> and run deconvolution in ImageJ instead, or have DeltaVision export to a
> format ImageJ understands.

Please note that Deltavision (*.dv) files are supported by Bio-Formats:

https://www.openmicroscopy.org/site/support/bio-formats/formats/deltavision.html

Regards,
-Melissa

On Thu, Dec 13, 2012 at 08:36:20AM -0500, Pariksheet Nanda wrote:

> On Sunday, December 9, 2012, Jasvinder S Ahuja wrote:
> >
> > 1) I cannot save the ROI as an image (.tiff file) On average I have 5-10
> > ROI
> > per image.
>
>
> Add your ROIs to the ROI manager. There are two ways to save the ROI
> information:
> - save to .roi file from the manager
> http://rsbweb.nih.gov/ij/docs/menus/analyze.html#manager
> - if you just save the .tiff image it should include the ROI drawn on the
> image as well, so you will see the ROI the next time you open it in ImageJ.
>
>
>
> > I want to save each ROI as an image in a format easy for ImageJ
> > to handle.
> >
>
> Using the .roi file explained above will accomplish this.
>
>
> 2) Also I am having problems opening deconvolved image files from
> > deltavision (*R3D_D3D.dv). Deltavision_opener plugin opens the non
> > deconvolved file (*R3D.dv). I believe this should be a simple fix but have
> > no clue, on how to go about it.
> >
>
>  I don't see support for that file with the LOI Bioformats plugin[1] so
> another approach could be to import the raw DeltaVision data into ImageJ
> and run deconvolution in ImageJ instead, or have DeltaVision export to a
> format ImageJ understands.
>
>
> Jasvinder
> >
>
>  Pariksheet
>
> [1] http://loci.wisc.edu/bio-formats/formats
>
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