http://imagej.273.s1.nabble.com/object-oriented-segmentation-tp5001110p5001156.html
2012/12/12 Straatman, Kees R. (Dr.) <
> Hi Michiel,
>
> Yes that is the result of using the nuclear image to segment the
> cytoplasmic signal. Not sure 1,2,3 how to improve that. You could try the
> watershed plugin from Daniel Saga (
>
http://bigwww.epfl.ch/sage/soft/watershed/) or Christopher Mei (
>
http://rsbweb.nih.gov/ij/plugins/watershed.html) to see if they give a
> better result for this.
>
> Best wishes
>
> Kees
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
> Michiel van Dommelen
> Sent: 12 December 2012 14:03
> To:
[hidden email]
> Subject: Re: object-oriented segmentation
>
> Hey Kees,
>
> Wauw! Bedankt! This is pretty amazing! Thanks!
> There is one thing that worries me a bit. I don’t know if this issue can
> be solved. In the attached image, you will find “Cytoplasm_Raw-1.jpg” in
> which the segmentation of the cells is visible. In red I have located some
> of the issues I am talking about. In these cases, a part of the cells is
> cut off, which results in an underestimation of cell area. Normally it
> would not be a big problem, but some of my cell treatments result in a
> shape change of these cells from “more round” to “more elongated”. And
> especially the elongated cells will be cut off more easily… Would you see a
> possible solution for this?
>
> Thanks,
> Michiel
> <
http://imagej.1557.n6.nabble.com/file/n5001136/Cytoplasm_Raw-1.jpg>
>
>
>
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