http://imagej.273.s1.nabble.com/I-need-to-copy-and-crop-mutiple-ROI-from-each-image-tp5000475p5001157.html
> To the question: How to save my ROI as image ?
>
> If what you want is just an illustration of the ROI used, you can always
> copy system them while they are superimposed to your image (Alt Gr + Print
> Screen).
>
> Alternatively, transform your tiff image to RGB, display your ROI on
> screen then in ROI manager window use the command Flatten (F), This will
> merge your ROI to the RGB image which you can later save as Tiff. I advise
> to have a thicker line to mark the edge of thr ROI si it is visible on the
> merged picture
>
> If all you want to get is your ROI on a transparent background, open your
> image, im the menu select:
> Image -> Stacks -> Add Slice
> Give the new slice a black background and display your ROI (again, thick
> line for the edges). Use Flatten (F) command and answer "No" when the
> software ask if you want to flatten all images. You will get your ROI on a
> black background. Save that image (either as jpg, bmp or other).
> Use an imaging software (photoshop or the gimp for example) and open the
> image. Using color select (or magic wand like method) select and remove all
> black from the picture so as to see your ROI without background. Save the
> image as png.
> Later when inserting it into power point or in web pages, you will have
> your ROI on transparent background which you will be able to superimpose to
> whatever you want.
>
> Cheers
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
> Pariksheet Nanda
> Sent: 13 December 2012 13:36
> To:
[hidden email]
> Subject: Re: I need to copy and crop mutiple ROI from each image
>
> On Sunday, December 9, 2012, Jasvinder S Ahuja wrote:
> >
> > 1) I cannot save the ROI as an image (.tiff file) On average I have
> > 5-10 ROI per image.
>
>
> Add your ROIs to the ROI manager. There are two ways to save the ROI
> information:
> - save to .roi file from the manager
>
http://rsbweb.nih.gov/ij/docs/menus/analyze.html#manager> - if you just save the .tiff image it should include the ROI drawn on the
> image as well, so you will see the ROI the next time you open it in ImageJ.
>
>
>
> > I want to save each ROI as an image in a format easy for ImageJ
> > to handle.
> >
>
> Using the .roi file explained above will accomplish this.
>
>
> 2) Also I am having problems opening deconvolved image files from
> > deltavision (*R3D_D3D.dv). Deltavision_opener plugin opens the non
> > deconvolved file (*R3D.dv). I believe this should be a simple fix but
> have
> > no clue, on how to go about it.
> >
>
> I don't see support for that file with the LOI Bioformats plugin[1] so
> another approach could be to import the raw DeltaVision data into ImageJ
> and run deconvolution in ImageJ instead, or have DeltaVision export to a
> format ImageJ understands.
>
>
> Jasvinder
> >
>
> Pariksheet
>
> [1]
http://loci.wisc.edu/bio-formats/formats>
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