ROI defined by two parallel lines

Posted by Oleg Broytman on Jan 12, 2013; 4:05am
URL: http://imagej.273.s1.nabble.com/ROI-defined-by-two-parallel-lines-tp5001382.html

Greetings.  I study airway wall remodeling and fibrosis in a rat model of
asthma.  My typical image is a transverse section of the rat lung, where I
can see a portion of the airway wall (not the full circle around the
airway, but just a sector of that circle).  To quantify the staining, I
would like to make an ROI of the airway wall by drawing two parallel
freehand lines, outlining the inside and outside border of the wall, and
defining the ROI as the area between those lines on 2 sides, and the top
and bottom of the image as the other 2 sides.


I have 2 questions:
- When I draw the first freehand line, and click the cursor somewhere else
to draw the second line, the first line disappears.  How can I avoid this?

- Once I have the two lines drawn, how do I define an ROI bound by lines
that don't connect to one another?

Thank you very much.

Oleg Broytman
Department of Medicine
University of Wisconsin - Madison

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