Re: deconvolution problem

Posted by bnorthan on Jan 15, 2013; 2:27am
URL: http://imagej.273.s1.nabble.com/deconvolution-problem-tp5001407p5001423.html

Hi Amy

My suggestion is to try DeconvolutionLab with the following settings:

Set the Algorithm to Richardson Lucy, set the iterations to 100, make
sure you set the PSF to the correct data set, then check "Flip PSF
quadrants" (if the PSF is centered).  I find you have to be careful
and make sure the image you want to deconvolve is focused before
opening the plugin.  When the deconvolution starts watch the output in
the log window carefully and verify it is loading the correct input
and psf and flipping the psf if needed.

With these settings you should be able to get an OK result, and at
least confirm it is working.   Then you could try Richardson-Lucy with
TV regularization and/or Thresholded Landweber.  Theoretically these
algorithms should give better results, at least with noisy images but
might require more adjusting of advanced paramaters.

Brian

On Mon, Jan 14, 2013 at 3:41 PM, Amy Cao <[hidden email]> wrote:

> Hi,
>
> Has anyone tried the deconvolution lab with ImageJ, with the dataset of
> fluorescent beads, which is provided by
> http://bigwww.epfl.ch/deconvolution/?p=bars ? I have tried, but I cannot
> get the result as they shows, I don't know why. Is there anyone can help?
>
> Thanks,
>
> Amy
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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