Re: deconvolution problem

Posted by Amy Cao on Jan 16, 2013; 7:43pm
URL: http://imagej.273.s1.nabble.com/deconvolution-problem-tp5001407p5001450.html

Hi Brian,

Thank you so much for your suggestion, I have made it according to your
suggestion. I think my problem is I did not flip PSF. But I don't
understand why I should flip PSF, do you have some idea on it?

Thanks,

Amy

On Mon, Jan 14, 2013 at 8:27 PM, Brian Northan <[hidden email]> wrote:

> Hi Amy
>
> My suggestion is to try DeconvolutionLab with the following settings:
>
> Set the Algorithm to Richardson Lucy, set the iterations to 100, make
> sure you set the PSF to the correct data set, then check "Flip PSF
> quadrants" (if the PSF is centered).  I find you have to be careful
> and make sure the image you want to deconvolve is focused before
> opening the plugin.  When the deconvolution starts watch the output in
> the log window carefully and verify it is loading the correct input
> and psf and flipping the psf if needed.
>
> With these settings you should be able to get an OK result, and at
> least confirm it is working.   Then you could try Richardson-Lucy with
> TV regularization and/or Thresholded Landweber.  Theoretically these
> algorithms should give better results, at least with noisy images but
> might require more adjusting of advanced paramaters.
>
> Brian
>
> On Mon, Jan 14, 2013 at 3:41 PM, Amy Cao <[hidden email]> wrote:
> > Hi,
> >
> > Has anyone tried the deconvolution lab with ImageJ, with the dataset of
> > fluorescent beads, which is provided by
> > http://bigwww.epfl.ch/deconvolution/?p=bars ? I have tried, but I cannot
> > get the result as they shows, I don't know why. Is there anyone can help?
> >
> > Thanks,
> >
> > Amy
> >
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>
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