Re: Problem with normalized variance for stack

Posted by sathya1 on Jan 31, 2013; 7:01pm
URL: http://imagej.273.s1.nabble.com/Problem-with-normalized-variance-for-stack-tp5001625p5001629.html

Thanks Michael! That helps a lot. I'm trying to detect focused images of isolated GFP-expressing cells within a stack. Right now, the normalized variance method works fairly decently. Its probably ~one frame off, but I will try your suggestion of the unsharp mask.