Posted by
ctrueden on
Feb 01, 2013; 8:15pm
URL: http://imagej.273.s1.nabble.com/Merge-Two-Videos-tp5001631p5001638.html
Hi Natalie,
> I have an nd2 file that I created by tracking two channels at once and
taking
> a picture twice per second over an amount of time. I'd like to make one
> channel show up in red and one in green, and I'd like to merge them
If your two channels are interleaved in the time dimension, you can split
them out by running Image > Properties (Ctrl+Shift+P) to edit the
dimensional lengths. Once you have a C slider that represents your two
channels, you can use Image > Color > Make Composite to generate a
red/green version (or other two-color combination; red/green is not good
for color-blind folks), as you describe.
The Bio-Formats Importer plugin's "Swap dimensions" option may also be
useful to you, depend on the way your image planes are ordered.
If you need further assistance, please feel free to upload a sample dataset
privately using Fiji's "Help > Upload Sample Image" command, and I can
determine the exact sequence of commands you would need to perform.
Note that ImageJ2 has new features to make this sort of thing easier: in
particular, an "Edit Axes" command that lets you reassign which axes are
which.
Regards,
Curtis
On Thu, Jan 31, 2013 at 8:30 PM, kegulian <
[hidden email]> wrote:
> Hello all, I have an nd2 file that I created by tracking two channels at
> once
> and taking a picture twice per second over an amount of time. I'd like to
> make one channel show up in red and one in green, and I'd like to merge
> them--I know how to do this with stills--so that each red picture stacks on
> top of each green one of the corresponding time point, creating a new
> merged
> stack (two-color video).
>
> No matter what I try, the video turns into just one picture, merged, losing
> the animation. Please help.
>
> Thank you.
>
>
>
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