Re: Merge Two Videos

Posted by kegulian on Feb 01, 2013; 9:17pm
URL: http://imagej.273.s1.nabble.com/Merge-Two-Videos-tp5001631p5001639.html

Hi Curtis,

Thanks for your reply. I already have the nd2 file as a stack in one window with a C-slider for channels and a Z-slider for time points. Then, I do Image > Color > Make Composite. But it just superposes one still from channel 0 over one still from channel 1. It doesn't do the same for all the images in the stack, only whichever image I'm on. Then, when I slide the Z-slider in the new window or press play, it just plays the one image, no animation.

There is no "Upload Sample Image" on my ImageJ Help menu. What is Fiji?

Regards,

Natalie

SAMPLES FROM MESSAGE BEING REPLIED TO

Curtis Rueden wrote

Hi Natalie,

If your two channels are interleaved in the time dimension, you can split
them out by running Image > Properties (Ctrl+Shift+P) to edit the
dimensional lengths. Once you have a C slider that represents your two
channels, you can use Image > Color > Make Composite to generate a
red/green version (or other two-color combination; red/green is not good
for color-blind folks), as you describe.

* * *

If you need further assistance, please feel free to upload a sample dataset
privately using Fiji's "Help > Upload Sample Image" command, and I can
determine the exact sequence of commands you would need to perform.

* * *

On Thu, Jan 31, 2013 at 8:30 PM, kegulian <[hidden email]> wrote:

> Hello all, I have an nd2 file that I created by tracking two channels at
> once
> and taking a picture twice per second over an amount of time. I'd like to
> make one channel show up in red and one in green, and I'd like to merge
> them--I know how to do this with stills--so that each red picture stacks on
> top of each green one of the corresponding time point, creating a new
> merged
> stack (two-color video).
>
> No matter what I try, the video turns into just one picture, merged, losing
> the animation. Please help.
>
> Thank you.
>
>
>
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