Posted by
Joel Sheffield on
Feb 01, 2013; 9:37pm
URL: http://imagej.273.s1.nabble.com/Merge-Two-Videos-tp5001631p5001640.html
Hi Natalie,
This depends on what your current arrangement, and the version of ImageJ
that you are using. Use the latest version of ImageJ. Depending on how
old your system is, you might want to reinstall the whole thing. However,
it seems as it you now have a "hyperstack" composed of the two sets of
images. If you go to Image>Hyperstacks>Hyperstack to Stack, you will now
have a single stack with alternating images. You can now go to
Image>Stack>Tools> Substack, and take a look at the instructions that pop
up. You should be able to make a substack containing just the one series.
If you click on "remove slices", the remaining stack will have the other of
the pair. Now, assuming that each stack is 8-bit, go to Image>Color>merge
colors, and select which stack you want as each color. The resultant
should be one stack with both images superimposed. (If these are different
from each other, you can look at them with red/green glasses, and see a 3D
image) If you are looking for colocalization, and want to convince people
like me, who are color blind, I suggest that you use a different pair.
Green/magenta works pretty well. Overlap appears white.
Joel
On Fri, Feb 1, 2013 at 4:17 PM, kegulian <
[hidden email]> wrote:
> Hi Curtis,
>
> Thanks for your reply. I already have the nd2 file as a stack in one window
> with a C-slider for channels and a Z-slider for time points. Then, I do
> Image > Color > Make Composite. But it just superposes one still from
> channel 0 over one still from channel 1. It doesn't do the same for all the
> images in the stack, only whichever image I'm on. Then, when I slide the
> Z-slider in the new window or press play, it just plays the one image, no
> animation.
>
> There is no "Upload Sample Image" on my ImageJ Help menu. What is Fiji?
>
> Regards,
>
> Natalie
>
> SAMPLES FROM MESSAGE BEING REPLIED TO
>
>
> Curtis Rueden wrote
> > Hi Natalie,
> >
> > If your two channels are interleaved in the time dimension, you can split
> > them out by running Image > Properties (Ctrl+Shift+P) to edit the
> > dimensional lengths. Once you have a C slider that represents your two
> > channels, you can use Image > Color > Make Composite to generate a
> > red/green version (or other two-color combination; red/green is not good
> > for color-blind folks), as you describe.
> >
> > * * *
> >
> > If you need further assistance, please feel free to upload a sample
> > dataset
> > privately using Fiji's "Help > Upload Sample Image" command, and I can
> > determine the exact sequence of commands you would need to perform.
> >
> > * * *
> >
> > On Thu, Jan 31, 2013 at 8:30 PM, kegulian <
>
> > NatalieCKegulian@
>
> > > wrote:
> >
> >> Hello all, I have an nd2 file that I created by tracking two channels at
> >> once
> >> and taking a picture twice per second over an amount of time. I'd like
> to
> >> make one channel show up in red and one in green, and I'd like to merge
> >> them--I know how to do this with stills--so that each red picture stacks
> >> on
> >> top of each green one of the corresponding time point, creating a new
> >> merged
> >> stack (two-color video).
> >>
> >> No matter what I try, the video turns into just one picture, merged,
> >> losing
> >> the animation. Please help.
> >>
> >> Thank you.
> >>
> >>
> >>
> >> --
> >> View this message in context:
> >>
http://imagej.1557.n6.nabble.com/Merge-Two-Videos-tp5001631.html> >> Sent from the ImageJ mailing list archive at Nabble.com.
> >>
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> >
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>
>
>
> --
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>
http://imagej.1557.n6.nabble.com/Merge-Two-Videos-tp5001631p5001639.html> Sent from the ImageJ mailing list archive at Nabble.com.
>
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--
Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail:
[hidden email]
URL:
http://astro.temple.edu/~jbs--
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