Posted by
ctrueden on
Feb 01, 2013; 9:35pm
URL: http://imagej.273.s1.nabble.com/Merge-Two-Videos-tp5001631p5001641.html
Hi Natalie,
> But it just superposes one still from channel 0 over one still from
> channel 1. It doesn't do the same for all the images in the stack,
> only whichever image I'm on.
How can you know that, until you move the Z or T slider to check?
> Then, when I slide the Z-slider in the new window or press play, it
> just plays the one image, no animation.
A "play" button (the little arrow) next to one of your sliders implies you
have more than one time point. In that case, ImageJ will animate over time,
not Z. It only animates over Z for single-time-point datasets.
Please run Image > Properties and ensure the dimensions are what you think
they should be. You should have 2 Channels, 1 Slice, and some number of
Frames greater than 1. The product of those three values should equal the
total number of planes in your dataset. If the numbers are wrong, you can
try typing in the correct values and see if that fixes your issues.
> What is Fiji?
Fiji is just ImageJ:
http://fiji.sc/It just comes with a bunch of extra plugins, and keeps them up to date for
you automatically. (This includes Bio-Formats, which you presumably must
have installed in order to get ImageJ to read your ND2 datasets.)
> There is no "Upload Sample Image" on my ImageJ Help menu.
If you install Fiji you will have the "Upload Sample Image" command in your
Help menu.
I am concerned that we may not actually understand what it is you want to
do. Sending a sample dataset may help to illustrate it more clearly.
Regards,
Curtis
On Fri, Feb 1, 2013 at 3:17 PM, kegulian <
[hidden email]> wrote:
> Hi Curtis,
>
> Thanks for your reply. I already have the nd2 file as a stack in one window
> with a C-slider for channels and a Z-slider for time points. Then, I do
> Image > Color > Make Composite. But it just superposes one still from
> channel 0 over one still from channel 1. It doesn't do the same for all the
> images in the stack, only whichever image I'm on. Then, when I slide the
> Z-slider in the new window or press play, it just plays the one image, no
> animation.
>
> There is no "Upload Sample Image" on my ImageJ Help menu. What is Fiji?
>
> Regards,
>
> Natalie
>
> SAMPLES FROM MESSAGE BEING REPLIED TO
>
>
> Curtis Rueden wrote
> > Hi Natalie,
> >
> > If your two channels are interleaved in the time dimension, you can split
> > them out by running Image > Properties (Ctrl+Shift+P) to edit the
> > dimensional lengths. Once you have a C slider that represents your two
> > channels, you can use Image > Color > Make Composite to generate a
> > red/green version (or other two-color combination; red/green is not good
> > for color-blind folks), as you describe.
> >
> > * * *
> >
> > If you need further assistance, please feel free to upload a sample
> > dataset
> > privately using Fiji's "Help > Upload Sample Image" command, and I can
> > determine the exact sequence of commands you would need to perform.
> >
> > * * *
> >
> > On Thu, Jan 31, 2013 at 8:30 PM, kegulian <
>
> > NatalieCKegulian@
>
> > > wrote:
> >
> >> Hello all, I have an nd2 file that I created by tracking two channels at
> >> once
> >> and taking a picture twice per second over an amount of time. I'd like
> to
> >> make one channel show up in red and one in green, and I'd like to merge
> >> them--I know how to do this with stills--so that each red picture stacks
> >> on
> >> top of each green one of the corresponding time point, creating a new
> >> merged
> >> stack (two-color video).
> >>
> >> No matter what I try, the video turns into just one picture, merged,
> >> losing
> >> the animation. Please help.
> >>
> >> Thank you.
> >>
> >>
> >>
> >> --
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> >>
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