Re: Densitometry guide (lines)?

Posted by jharford on Feb 05, 2013; 5:19pm
URL: http://imagej.273.s1.nabble.com/Densitometry-guide-lines-tp3698971p5001676.html

"Are there any guidelines how to define peaks and to integrate them, esp. when there is no baseline separation of bands or the baseline is sort of skew?"

Another possible solution would be to use LI-COR's free Image Studio Lite software (www.licor.com/islite) to analyze your western blot data from the Odyssey.  They have quickstart guides, tutorials and videos on how to define bands and integrate them, but it is pretty easy to use.