isosurfacing of 2P fluorescence images

Posted by Yu, Alex (NIH/NICHD) [F] on Feb 06, 2013; 1:17am
URL: http://imagej.273.s1.nabble.com/how-can-I-call-execute-run-a-trained-weka-classifier-from-an-ImageJ-macro-tp5001668p5001683.html

hi,
I have a bunch of images to be processed with cell membrane iso-surfacing. All these images were captured from FP labelled cells under Leica SP5 2P microscope. The boundaries of some of membrane structures such as vesicle/vacuoles are a little bit blur compared to plasma membrane. Is there any plugin available by which I can do isosurfacing of both PM and intracellular vesicle/vacuoles?  
Many thanks,
ALex
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