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Re: Using Image J to count live and dead cells in cultures

Posted by Shall, Sydney on Feb 05, 2013; 4:34pm
URL: http://imagej.273.s1.nabble.com/Using-Image-J-to-count-live-and-dead-cells-in-cultures-tp3686899p5001685.html

I would be inclined to use a double staining technique; perhaps and also
a non-vital dye with a different spectrum.
Then count the number of cells with each colour.
Sydney



On 05/02/2013 04:44, pmbcellcounter wrote:

> *bump*
>
> Surprise! I am facing the exact similar situation in 2013!
>
> Can anyone help? Thanks in advance...
>
>
>
> --
> View this message in context: http://imagej.1557.n6.nabble.com/Using-Image-J-to-count-live-and-dead-cells-in-cultures-tp3686899p5001666.html
> Sent from the ImageJ mailing list archive at Nabble.com.
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
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--
Professor Sydney Shall,
Department of Haematological Medicine,
King's College London,
Medical School,
123 Coldharbour Lane,
LONDON SE5 9NU,
Tel & Fax: +44 (0)207 848 5902,
E-Mail: sydney.shall,
[correspondents outside the College should add; @kcl.ac.uk]
www.kcl.ac.uk

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