RGB automation

Posted by Brenda on
URL: http://imagej.273.s1.nabble.com/RGB-automation-tp5001706.html

Hello everyone. I'm looking for some advice for automating image processing for a big batch of 3 color fluorescent images. I'm imaging red, green or blue fluorescence as a single channel. The output of the microscope's software is in RGB color, therefore 1 channel has information and the other 2 channels are blank. Quirk of the software and I'm stuck with it. I have been manually doing a RGB split, closing the blank channels, and manually RGB merge.

Measuring the intensity of the data image gives me a number (345671) while measuring the blank ones gives me zero.

Any ideas on how to put that in a macro?

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html