How to save a file in LSM format in ImageJ?
Posted by mia2005 on Feb 11, 2013; 4:22pm
URL: http://imagej.273.s1.nabble.com/How-to-save-a-file-in-LSM-format-in-ImageJ-tp5001719.html
Hello all,
I am acquiring my Zstack images with a Leica LSM710 using the program ZEN
that saves the images in LSM format. Then I want to measure co-localization
of 2 out of my 4 channes with IMARIS.
However, prior to my analysis in IMARIS, I would like to edit them in ImageJ
to delete some areas (by using edit>clear selection, I can delete the
nucleus out of my analysis plane for example). However, after I do this, I
am not able to save this file in a format that I can open it in IMARIS. I
tried several formats, and the ones that do open something, only one channel
shows up instead of the 4.
Does anyone know how to do this?
Thank you very much