Re: How to save a file in LSM format in ImageJ?

Posted by ctrueden on Feb 11, 2013; 5:33pm
URL: http://imagej.273.s1.nabble.com/How-to-save-a-file-in-LSM-format-in-ImageJ-tp5001719p5001720.html

Hi,

> I am not able to save this file in a format that I can open it in
> IMARIS. I tried several formats, and the ones that do open something,
> only one channel shows up instead of the 4.

I advise you not to resave in a proprietary file format like Zeiss LSM. Use
an open file format such as OME-TIFF. The Bio-Formats Exporter plugin for
ImageJ (bundled with Fiji) can save as OME-TIFF.

Imaris can open OME-TIFF files [1]. If you have problems, you can report
them to Bitplane.

Alternately, Imaris also has integration with Fiji [2], which could allow
you to perform your ImageJ-based workflow directly from within Imaris.

Regards,
Curtis

[1] http://www.bitplane.com/go/products/imaris, "Datasheet" tab, "Supported
formats" section
[2]
http://www.bitplane.com/go/knowledge-base/how-to--/enabling-imagej/fiji-and-matlab-plugins-in-imaris

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