> Hi Thuraya,
>
> > while um doing the order "fill holes" this is the image i got
>
> I was able to essentially duplicate your (erroneous) result by first
> running the "Invert LUT" command before running Fill Holes. So it seems the
> Fill Holes command is sensitive to the LUT somehow.
>
> Is your Fiji fully up to date? (Help > Update Fiji)
>
> When I run the "Create Mask" command with a fully up to date Fiji on OS X,
> I receive a Mask window with "8-bit (inverting LUT)" in the title area. I
> notice in your screenshot that your LUT is not inverted like that. So I
> suspect your error has something to do with the LUT. Are you inverting it
> manually? Or do you have the "Use inverting lookup table" option in Edit >
> Options > Appearance checked? (That option is off for me.)
>
> If you are stumped, you can probably avoid the issue by running "Invert
> LUT" followed by "Fill Holes" followed by "Invert LUT" again. Though
> ideally it would be best to learn why the problem is happening, of course.
>
> Regards,
> Curtis
>
> P.S. Note that it is also possible for the "Fill Holes" command to behave
> unexpectedly if you have many dense particles that are all connected in a
> large structure. The command interprets it as one giant structure which
> needs filling in. So if you used a substantially wider Brightness setting
> than 0-180 with your image, you may experience this phenomenon as well. In
> short, try to get your particles well-separated when possible.
>
>
> On Wed, Feb 20, 2013 at 12:06 PM, Thuraya Medhat
> <
[hidden email]>wrote:
>
> > >
> > > it's me again um realy sorry for bothering but while um doing the
> order''
> > > fill holes'' this is the image i got idunnu wt's gone wrong
> > >
> >
> >
> > >
http://img10.imageshack.us/img10/3083/14615797.png> > >
> >
> >
> > >
> > > On Mon, Dec 10, 2012 at 11:50 PM, Curtis Rueden <
[hidden email]>
> > wrote:
> > >
> > >> Hi Thuraya,
> > >>
> > >> > could you please do a screen shot about what you mean when you said
> > >> > "- Click the Select button in the Threshold Color dialog to create a
> > >> > selection''
> > >>
> > >> Look again at the screenshot I already linked:
> > >>
> > >>
http://code.imagej.net/curtis/fiji-particles.jpg> > >>
> > >> You see the dialog box entitled "Threshold Color"? There is a button
> at
> > >> the
> > >> bottom called "Select", which is what I was referring to. This button
> > >> creates a selection based on the defined threshold, which you can then
> > >> convert to a mask, etc.
> > >>
> > >> -Curtis
> > >>
> > >>
> > >> On Mon, Dec 10, 2012 at 2:56 PM, Thuraya Medhat <
> >
[hidden email]
> > >> >wrote:
> > >>
> > >> > Dear curties,
> > >> > you realy did a WONDERFUL job,, just need one thing you told me to
> do
> > >> > selection before i do the mask option
> > >> > but that doesn't work with me could you please do a screen shot
> about
> > >> what
> > >> > you mean when you said "- Click the Select button in the Threshold
> > Color
> > >> > dialog to create a selection''
> > >> >
> > >> > On Mon, Dec 10, 2012 at 8:41 PM, Curtis Rueden <
[hidden email]>
> > >> wrote:
> > >> >
> > >> > > - Click the Select button in the Threshold Color dialog to create
> a
> > >> > > selection
> > >> > >
> > >> >
> > >> > --
> > >> > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> > >> >
> > >>
> > >> --
> > >> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> > >>
> > >
> > >
> >
> > --
> > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> >
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>