Turboreg: Problem with jpg and png image format

Posted by JoLi on Mar 06, 2013; 5:56pm
URL: http://imagej.273.s1.nabble.com/Turboreg-Problem-with-jpg-and-png-image-format-tp5002040.html

Hello everybody,

I have a problem I can't figure out alone any more. Out of my microscope data I create image files using mathlab. with turboreg I want to align the images with respect to another. This works perfekt when I create .jpg files with mathlab, but if I use .png or even .tif images the error "At least two grayscale or RGB-stack images are required".
I tried to get rid of this problem changing the export options in mathlab to grayscale, but it doesn't work. This option is also under the "rendering" dialog box, so i am even not sure if this is the right place to change the export options but I didn't finde any other options.

I want to use .png images because it is a lossles compression and .jpg smoothes my data, though changing it a bit which is not wanted. My data is only in grayscale, but the images seem to appear as colour, what I don't understand. I would be very happy if anybody has some ideas.

Thank you.

Jonas