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Re: Turboreg: Problem with jpg and png image format

Posted by Joel Sheffield on Mar 07, 2013; 3:36pm
URL: http://imagej.273.s1.nabble.com/Turboreg-Problem-with-jpg-and-png-image-format-tp5002040p5002049.html

Hi Jonas,

Maybe, even though you convert to grayscale using mathlab, the program
saves the file in an rgb format.  You can convert to an 8-bit grayscale
image in ImageJ by loading the images, and selecting Image>type, and
selecting 8-bit grayscale as an option.

Joel


On Wed, Mar 6, 2013 at 12:56 PM, JoLi <[hidden email]>wrote:

> Hello everybody,
>
> I have a problem I can't figure out alone any more. Out of my microscope
> data I create image files using mathlab. with turboreg I want to align the
> images with respect to another. This works perfekt when I create .jpg files
> with mathlab, but if I use .png or even .tif images the error "At least two
> grayscale or RGB-stack images are required".
> I tried to get rid of this problem changing the export options in mathlab
> to
> grayscale, but it doesn't work. This option is also under the "rendering"
> dialog box, so i am even not sure if this is the right place to change the
> export options but I didn't finde any other options.
>
> I want to use .png images because it is a lossles compression and .jpg
> smoothes my data, though changing it a bit which is not wanted. My data is
> only in grayscale, but the images seem to appear as colour, what I don't
> understand. I would be very happy if anybody has some ideas.
>
> Thank you.
>
> Jonas
>
>
>
>
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>
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--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs

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