Re: quantification of DAB staining

Posted by carlo bianco on
URL: http://imagej.273.s1.nabble.com/quantification-of-DAB-staining-tp5002115p5002116.html

hi
you can try the color deconvolution way ( landini's plugin)
or the color segmentation,
moreover, is available online a software developed for ki-67 nuclear staining
quantification http://breast-cancer-research.com/content/12/4/R56
maybe at 40 x magnification it could work.
Let me know
Good luck

carlo  bologna Italy




________________________________
 Da: rona baron <[hidden email]>
A: [hidden email]
Inviato: Martedì 12 Marzo 2013 10:20
Oggetto: quantification of DAB staining
 
Hi all,

I stained tissues with antibodies specific for neurons (MAP-2) and with
hematoxylin which stains all the nucleus population, than imaged it with
light microscope.
Is it possible to distinguish between the brown staining for neurons and
the purple staining for the nucleus?
I would like to quantify the number of brown particles that I have out of
the total purple particles....


Thanks,
Rona

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