Re: quantification of DAB staining

Posted by Shall, Sydney on Mar 12, 2013; 10:49am
URL: http://imagej.273.s1.nabble.com/quantification-of-DAB-staining-tp5002115p5002118.html

Dear Rona,
There is a plug-in for ImageJ crafted by Dr Pawel Znojek, which
essentially aims to do this.
He constructed it for DAPI and a fluorescent  antibody to a DNA repair
protein.
You should be able to adapt this easily for your purposes.
The plugin is available from the following site:
http://www.pzfociez.com/ .
I would recommend that you should add a plugin constructed by Gabriel
Landini, at the University of Birmingham, England, which helps considerably.
This you can find on the ImageJ site.

If you proceed down this path, I should be very grateful to you if you
could let me know how things develop for you.

With best wishes,

Sydney



On 12/03/2013 09:20, rona baron wrote:

> Hi all,
>
> I stained tissues with antibodies specific for neurons (MAP-2) and with
> hematoxylin which stains all the nucleus population, than imaged it with
> light microscope.
> Is it possible to distinguish between the brown staining for neurons and
> the purple staining for the nucleus?
> I would like to quantify the number of brown particles that I have out of
> the total purple particles....
>
>
> Thanks,
> Rona
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

--
Professor Sydney Shall,
Department of Haematological Medicine,
King's College London,
Medical School,
123 Coldharbour Lane,
LONDON SE5 9NU,
Tel & Fax: [phone],
E-Mail: sydney.shall,
[correspondents outside the College should add; @kcl.ac.uk]
www.kcl.ac.uk


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