quantification of fluorescent signal per a cell

Posted by Junsung LEE on Mar 13, 2013; 2:22am
URL: http://imagej.273.s1.nabble.com/quantification-of-fluorescent-signal-per-a-cell-tp5002137.html

I want to quantify fluorescent signal (punctiform, like cellular vesicles) in one cell, but I don't know how. I have tried a few things as follows (but all of them were not successful) :


1. first, I selected one cell with 'polygon selections tool', and tried to analyse fluorescent signal with 'RGB profile (Plugins>Graphics>RGB profile)'. But I failed because 'RGB profile' is working only with 'line or rectangular selections tool'. I can apply 'rectangular selections tool' in some cases, but when cells are overlapped partially, it is not applicable.

> is there any ways to make 'RGB profile' work with 'polygon selections tool' or 'freehand selections tool'?


2. second, I marked fluorescent signals (they are dot-shaped, as I said above) with 'multi-point selections tool' one by one, and analysed them with 'measure (analyse>measure (ctrl+M)'. This method is working but marking many fluorescent dots one by one is really hard.

> I really wonder how to select fluorescent signals (dots) automatically. Once I designate one dot as signal, it is possible to select dots automatically, I think, but I don't know how.


I will be appreciated if you tell me how. If appropriate method is exist except what I said above, please don't hesitate to   let me know. Please refer to attached image file.

Thanks in advance.


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