Issue with the brightness/contrast of my 16-bit TIFF stack

Posted by Charison on
URL: http://imagej.273.s1.nabble.com/Issue-with-the-brightness-contrast-of-my-16-bit-TIFF-stack-tp5002427.html

Dear all,

I am a student currently working with ImageJ. After converting my 32-bit real TIFF image stack to the 16-bit unsigned TIFF image stack using the Image > Type option, I find that the new 16-bit image stack has varying levels of brightness, different from the original image stack. Can anyone help me with this?

Thanks