Posted by
Stoyan Pavlov on
Mar 26, 2013; 12:49pm
URL: http://imagej.273.s1.nabble.com/GUS-staining-quantification-tp5002411p5002428.html
Hello Magi,
Can you attach a sample image?
When I was starting with image acquisition and analysis I had similar
problem (i guess): My white balance was off somehow and the acquired images
of DyI stained tissue were oversaturated in the red channel, however in the
green the dynamic range was just fine, so I used the green channel instead.
As long as there are no multiple labels visible while you acquire the image
you can easily use another channel (still all your images must be acquired
the same way and you'll need to use the same channel in order to be able to
compare them).
Stoyan
2013/3/25 magi78343 <
[hidden email]>
> Dear all,
>
> I need to quantify the GUS-staining intensity (blue) of some digital
> images.
> As far as I know I first need to split the RGB channels. I am interested
> only in the blue intensity but the blue channel is the pilest one and the
> dark gray color corresponding to the dark blue on the color image remains
> in
> the red channel. Which channel should I use for measurement?
>
> Thank you in advance.
>
> Magi
>
>
>
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Dr. Stoyan P. Pavlov, MD, PhD
Departament of Anatomy, Histology and Embryology
Medical University "Prof. Dr. Paraskev Stoyanov", Varna
Prof. Marin Drinov Str.55
9002 Varna
Bulgaria
Tel: +359 (0) 52 - 677 - 086
e-mail:
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