Re: GUS staining quantification

Posted by G. Esteban Fernandez on Mar 26, 2013; 7:54pm
URL: http://imagej.273.s1.nabble.com/GUS-staining-quantification-tp5002411p5002447.html

Hi Magi,

I would convert from RGB to HSB (Hue, Saturation, Brightness) and measure
the intensity of the Saturation channel.

Generally, Saturation reflects the amount of dye present.  Brightness
depends more by the density of the tissue and Hue = Color (since you have
one consistent dye/color the Hue doesn't change).

-Esteban


On Tue, Mar 26, 2013 at 11:49 AM, magi78343 <[hidden email]> wrote:

> Hi Sergey,
> Here is a nice example of the images I'm working with.  I have to measure
> the mean intensity of the whole blue area in the tip by drawing ROI around
> it (my supervisor doesn't like the tresholding)
>
> <
> http://imagej.1557.n6.nabble.com/file/n5002445/AUX1-GUS_1%C2%B5M_ACC_8.jpg
> >
>
> Best,
> Magi
>
>
>
>
>
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