Posted by G. Esteban Fernandez on Mar 26, 2013; 7:56pm URL: http://imagej.273.s1.nabble.com/GUS-staining-quantification-tp5002411p5002448.html
You can convert to HSB with: Image > Type > HSB Stack.
-Esteban
On Tue, Mar 26, 2013 at 12:54 PM, G. Esteban Fernandez <
[hidden email]> wrote:
> Hi Magi,
>
> I would convert from RGB to HSB (Hue, Saturation, Brightness) and measure
> the intensity of the Saturation channel.
>
> Generally, Saturation reflects the amount of dye present. Brightness
> depends more by the density of the tissue and Hue = Color (since you have
> one consistent dye/color the Hue doesn't change).
>
> -Esteban
>
>
> On Tue, Mar 26, 2013 at 11:49 AM, magi78343 <[hidden email]> wrote:
>
>> Hi Sergey,
>> Here is a nice example of the images I'm working with. I have to measure
>> the mean intensity of the whole blue area in the tip by drawing ROI around
>> it (my supervisor doesn't like the tresholding)
>>
>> <
>> http://imagej.1557.n6.nabble.com/file/n5002445/AUX1-GUS_1%C2%B5M_ACC_8.jpg >> >
>>
>> Best,
>> Magi
>>
>>
>>
>>
>>
>> --
>> View this message in context:
>> http://imagej.1557.n6.nabble.com/GUS-staining-quantification-tp5002411p5002445.html >> Sent from the ImageJ mailing list archive at Nabble.com.
>>
>> --
>> ImageJ mailing list: http://imagej.nih.gov/ij/list.html >>
>
>