Re: GUS staining quantification

Posted by magi78343 on Mar 27, 2013; 11:03am
URL: http://imagej.273.s1.nabble.com/GUS-staining-quantification-tp5002411p5002455.html

Hello again,
Thank you all for your advices.

As I try to avoid tresholding I decided to use the Red channel from the RGB stack. I don't want the results to be in gray values so I calibrated all images to a step-tablet (manual for the calibration: http://imagej.nih.gov/ij/docs/examples/calibration/). Now the results I get are in OD. Is the measure ment ok  like this or I should better use other way of calibration?

Best regards,
Magi