Re: GUS staining quantification

Posted by G. Esteban Fernandez on Mar 27, 2013; 7:32pm
URL: http://imagej.273.s1.nabble.com/GUS-staining-quantification-tp5002411p5002464.html

You can compute OD from the intensity value:  OD = log(1/transmittance),
where transmittance = intensity/max. possible intensity

-Esteban


On Wed, Mar 27, 2013 at 4:03 AM, magi78343 <[hidden email]> wrote:

> Hello again,
> Thank you all for your advices.
>
> As I try to avoid tresholding I decided to use the Red channel from the RGB
> stack. I don't want the results to be in gray values so I calibrated all
> images to a step-tablet (manual for the calibration:
> http://imagej.nih.gov/ij/docs/examples/calibration/). Now the results I
> get
> are in OD. Is the measure ment ok  like this or I should better use other
> way of calibration?
>
> Best regards,
> Magi
>
>
>
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