Posted by
Michael Schmid on
Apr 26, 2013; 1:15pm
URL: http://imagej.273.s1.nabble.com/analyze-particles-exclude-holes-tp5002812p5002814.html
Hi Helmut,
assuming you particles are not embedded in a frame, which would make it one big particle, the size of the frame (this is hard to see in your thumbnails):
The measurements are done correctly:
If I convert your image with thick outlines to 8-bit-grayscale, threshold it and run 'Analyze particles' with 'include holes' unchecked, I get the result for the thick outlines only. E.g. the 'Mean' brightness is about 10 (very dark), maximum brightness is 127 or 129 (the threshold).
If I select 'include holes', the maximum brightness is 255 (white in the interior) and the 'Mean' brightness is in the 66-104 range.
You did not say it, but I guess that your second image is the result of 'show outlines'?
Well, that's a question what you define as 'Outline'.
Wikipedia says, "Outline drawing" is "a sketch depicting the outer edges of a person or object, without interior details or shading". So one would not expect interior edges, I guess?
If you select 'Masks' as output, you will see the area that actually gets analyzed.
Michael
________________________________________________________________
On Apr 26, 2013, at 14:45, Helmut Bergen wrote:
> Hello
>
> I try to do analyze particles and don't understand why ImageJ don't' exclude
> holes, although I didn't check the "include holes" button.
>
> For example this picture.
>
> And the result is:
>
> Where is the mistake???
>
>
>
> Thanks for helping and best wishes
>
> Helmut
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