> Hello Yuan Chen,
>
> it is possible to preserve the grayvalues when thresholding. But you have
> to convert your 16-bit images to 32-bit. Then run the thresholding command
> and IJ will ask you to "Set Background Pixels to NaN". When you use a macro
> the commands
> > setAutoThreshold("Default dark"); // or select your favourite method
> > run("NaN Background");
> will do the trick.
>
> Best regards
> Michael Epping
>
> Am 28.04.2013 21:08, schrieb Yuan Chen:
>
>> Dear all friends
>>
>> I am now trying to threshold my 16 bits tiff image sequence by grayscale
>> value. This was done by using imageJ ' threshold' command. But this seems
>> can only allow you to save images as binary. Say pixel that about GV 24340
>> will be 1 , the rest of them will be 0.
>>
>> Is there any way to threshold images, but still save them as the 16 bits
>> images ( with the graycalue ranged 0 -65525) :)
>>
>> Best wishes
>>
>> Yuan Chen
>>
>> University of Sheffield
>>
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