On 29 April 2013 09:16, Straatman, Kees R. (Dr.) <
> Dear Yuan Chen,
>
> Alternatively, you could try the macro code below.
>
>
> title1 = getTitle;
> getDimensions(width, height, channels, slices, frames);
> run("Duplicate...", "title=[duplicate] duplicate range=1-"+slices);
> run("Threshold...");
> title = "Message";
> msg="Set threshold then click \"OK\"";
> waitForUser(title, msg);
> run("Convert to Mask");
> setBatchMode(true);
> run("Analyze Particles...", "size=1-Infinity circularity=0.00-1.00
> show=Nothing add stack");
> for (i=0;i<roiManager("count");i++){
> selectWindow(title1);
> roiManager("Select", i);
> run("Copy");
> selectWindow("duplicate");
> roiManager("Select", i);
> run("Paste");
> }
> run("Select None");
> roiManager("Show None");
> setBatchMode("exit and show");
>
> Best wishes
>
> Kees
>
>
> Dr Ir K.R. Straatman
> Senior Experimental Officer
> Centre for Core Biotechnology Services
> University of Leicester
>
http://www.le.ac.uk/biochem/microscopy/home.html>
>
>
> -----Original Message-----
> From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of
> Yuan Chen
> Sent: 28 April 2013 20:08
> To:
[hidden email]
> Subject: About thresholding
>
> Dear all friends
>
> I am now trying to threshold my 16 bits tiff image sequence by grayscale
> value. This was done by using imageJ ' threshold' command. But this seems
> can only allow you to save images as binary. Say pixel that about GV 24340
> will be 1 , the rest of them will be 0.
>
> Is there any way to threshold images, but still save them as the 16 bits
> images ( with the graycalue ranged 0 -65525) :)
>
> Best wishes
>
> Yuan Chen
>
> University of Sheffield
>
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