Posted by
ctrueden on
May 03, 2013; 6:38pm
URL: http://imagej.273.s1.nabble.com/3D-Viewing-with-multiple-channels-tp5002914p5002915.html
Hi Celeste,
> I am struggling with opening a multichannel, multi-z .lsm into 3D
> Viewer and getting multiple channels.
Bene Schmid, the author of the 3D Viewer, may have other suggestions, but
here is one way:
1. Open your data as a normal hyperstack.
2. Run Image > Color > Make Composite and ensure it is in Composite mode.
3. Optionally, use the Image > Lookup Tables for each channel to assign the
desired color table to each channel.
4. Flatten the channels using Image > Type > RGB Color (note: this will
force each channel to 8-bit, which may be lossy depending on your data)
5. Run Plugins > 3D Viewer to get a color 3D visualization.
Regards,
Curtis
On Fri, May 3, 2013 at 12:45 PM, Celeste Eno <
[hidden email]> wrote:
> Hello,
> Thank you in advance. I am sure this is quite easy, but I am struggling
> with opening a multichannel, multi-z .lsm into 3D Viewer and getting
> multiple channels. I also tried Image5D, but I must not be doing the
> correct things.
>
> How do I open multi-channel, Z-stack so as to look at a 3D rendering?
>
> Thank you,
> Celeste Eno
>
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