Re: using a cheap ccd cam to capture on transilluminator
Posted by
Gabriel Landini on
May 17, 2013; 8:14am
URL: http://imagej.273.s1.nabble.com/using-a-cheap-ccd-cam-to-capture-on-transilluminator-tp5003028p5003039.html
[hidden email]> wrote:
> Hi everyone,
>
> this is probably a little bit off topic, but since this is the best
> audience of camera/imaging experts I know I will try to ask here...
> I was trying to use ImageJ to acquire images of my gels/plates that sits
> on transilluminator (both white light or UV) and I realized that when I
> try
> to do that a strange pattern of lines appears on the image. Initially I
> was
> thinking that the reason was that I was using a very cheap CCD (a 10 $
> webcam), but the same pattern appears if I take a picture with my phone
> camera. The pattern seems depending on the chip, because if I turn the
> camera it turns as well. Does any one have an explanation for this? More
> importantly there is a way around it.
> The idea is essentially to image directly on my computer without using a
> 10 K$ expensive gel doc
Without posting an image of the artifacts it is impossible to say, but in
general, you get what you pay for.
Do not know if that is the case here, but many cheap cameras do progressive
scans. So if your light source flickers (like in LEDs intensity controlled by
pulse width modulation or fluorescent tubes) then you might get illumination
artifacts.
Cheers
Gabriel
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