Using overlays on image channels

Posted by edsimmons on May 20, 2013; 11:45am
URL: http://imagej.273.s1.nabble.com/Using-overlays-on-image-channels-tp5003054.html

Hi IJ list,

I wonder if someone could help, please?

I'm trying to work out how best to use overlays on an image with two channels. I'm using Micro-manager to acquire images (displayed as greyscale channels that the user can choose between, not a composite image) and I would like to draw on the overlays of individual channels of the same image.

Is this something that is possible to do with IJ directly or do I need to code this myself?

I recall that it can be made to work the way I want with stacks of images (eg each image in the stack has it's own overlay) but I can't make this happen using channels.

Any help gratefully receieved - thanks in advance.

Best regards,
Ed