Re: Shading correction

Posted by G. Esteban Fernandez on Jun 26, 2013; 8:16pm
URL: http://imagej.273.s1.nabble.com/Shading-correction-tp5003667p5003661.html

Use Image Calculator (bare ImageJ) or Calculator Plus (Fiji) and perform a
divide operation between each tile and your shading reference image.  This
won't work if you already stitched all of your tiles together, in which
case you'll have to generate a stitched reference image.

In Image Calculator generate a 32-bit result.  In Calculator Plus multiply
by a konstant that is near the average pixel intensity of the tile.

-Esteban
On Jun 26, 2013 1:03 PM, "Fabiano Perina" <[hidden email]> wrote:

> Hello all,
>
> I'm kind a new ImageJ (Fiji) user and I wonder if you can help me.
>
> I am trying to do shading correction on tiled  images generated by a High
> Speed
> Confocal microscope. So does anyone know a plugin or macro to do this job
> using my reference image taken at the same conditions used for the multi
> tile images?
>
> Since now I thank for your attention and time!
> Best regards, Perina.
>
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