Re: Shading correction

Posted by Rob van 't Hof-2 on
URL: http://imagej.273.s1.nabble.com/Shading-correction-tp5003667p5003666.html

Hi,
use the shading corrector plugin from
http://rsbweb.nih.gov/ij/plugins/shading-corrector.html
or use my variant which prevents weird effects of overflow of 8-bit
values, and performs the colour shading by converting the image to HSB
format and does the correction on the B (brightness) band and leaves the
hue and saturation as they are. for me that often works better.

You can get my variant at:
https://dl.dropboxusercontent.com/u/50702312/Shading_Corrector_RvH.class
https://dl.dropboxusercontent.com/u/50702312/Shading_Corrector_RvH.java

Both variant look for a reference image with the name "blank-field", so
rename your reference image like this.

Hope this helps,
Rob

On 26/06/2013 20:49, Fabiano Perina wrote:

> Hello all,
>
> I'm kind a new ImageJ (Fiji) user and I wonder if you can help me.
>
> I am trying to do shading correction on tiled  images generated by a High Speed
> Confocal microscope. So does anyone know a plugin or macro to do this job using my reference image taken at the same conditions used for the multi tile images?
>
> Since now I thank for your attention and time!
> Best regards, Perina.
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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Dr. Rob van 't Hof
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Centre for Molecular Medicine
MRC IGMM
University of Edinburgh
Western General Hospital
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United Kingdom

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