Re: Shading correction

Posted by pang on
URL: http://imagej.273.s1.nabble.com/Shading-correction-tp5003667p5003678.html

Esteban and Fabiano,

In Image Calculator generate a 32-bit result.  In Calculator Plus multiply by a konstant that is near the average pixel intensity of the tile.
Why don't you multiple a constant that is the maximum of your reference image (smoothed). I think it is better than using the average.

Zhengyu

Zhengyu Pang, Ph.D.
Biochemistry and Bioanalytics Laboratory
Diagnostic and Biomedical Technologies
GE Global Research, K1-5B37A
One Research Circle
Niskayuna, NY 12309
T: 518-387-4015
F: 518-387-7765



-Esteban
On Jun 26, 2013 1:03 PM, "Fabiano Perina" <[hidden email]> wrote:

> Hello all,
>
> I'm kind a new ImageJ (Fiji) user and I wonder if you can help me.
>
> I am trying to do shading correction on tiled  images generated by a
> High Speed Confocal microscope. So does anyone know a plugin or macro
> to do this job using my reference image taken at the same conditions
> used for the multi tile images?
>
> Since now I thank for your attention and time!
> Best regards, Perina.
>
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