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Re: Shading correction

Posted by G. Esteban Fernandez on Jun 27, 2013; 3:36pm
URL: http://imagej.273.s1.nabble.com/Shading-correction-tp5003667p5003679.html

You're right, I use average for transmitted light images (brightfield) and
max for fluorescence images. Thanks for the correction.

-Esteban
On Jun 27, 2013 6:55 AM, "Pang, Zhengyu (GE Global Research)" <[hidden email]>
wrote:

> Esteban and Fabiano,
>
> In Image Calculator generate a 32-bit result.  In Calculator Plus multiply
> by a konstant that is near the average pixel intensity of the tile.
> Why don't you multiple a constant that is the maximum of your reference
> image (smoothed). I think it is better than using the average.
>
> Zhengyu
>
> Zhengyu Pang, Ph.D.
> Biochemistry and Bioanalytics Laboratory
> Diagnostic and Biomedical Technologies
> GE Global Research, K1-5B37A
> One Research Circle
> Niskayuna, NY 12309
> T: 518-387-4015
> F: 518-387-7765
>
>
>
> -Esteban
> On Jun 26, 2013 1:03 PM, "Fabiano Perina" <[hidden email]> wrote:
>
> > Hello all,
> >
> > I'm kind a new ImageJ (Fiji) user and I wonder if you can help me.
> >
> > I am trying to do shading correction on tiled  images generated by a
> > High Speed Confocal microscope. So does anyone know a plugin or macro
> > to do this job using my reference image taken at the same conditions
> > used for the multi tile images?
> >
> > Since now I thank for your attention and time!
> > Best regards, Perina.
> >
> > --
> > ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> >
>
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