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Re: I may have found the cause of my problem stitching stacks with 5 planes...

Posted by Stephan Preibisch on Jul 05, 2013; 9:08pm
URL: http://imagej.273.s1.nabble.com/I-may-have-found-the-cause-of-my-problem-stitching-stacks-with-5-planes-tp5003778p5003781.html

Hi Laurent,

I suspect that something may go wrong when loading the images. Do you use
TIFFs?

Nice greetings,
Stephan

> Hi everybody,
>
>
> Following my last e-mail (see below), I looked at
> http://fiji.sc/Image_Stitching#Problems.2C_known_issues_and_solutions
> (thanks to Pariksheet Nanda) and it is stated that Z-stacks should have at
> least 3 planes. I tested different numbers (adding empty planes to my
> stacks), and actually any number equal to or above 8 seem fine. I guess
> this is because it is now possible to merge 7 channels...??
>
> If this is the answer, then I would find nice, provided that image
> properties are set correctly, if the plugin could not convert Z-planes
> into channels. It does happen to make Z-stacks with less than 8 planes...
>
>
>
> Many thanks in advance for your help,
>
>
>
> Best regards (and have a nice week-end),
>
>
>
> Laurent.
>
>
>
> From: Gelman, Laurent
> Sent: 05 July 2013 13:28
> To: '[hidden email]'
> Subject: Stitching of Z-stacks converts Z planes into color channels. Is
> this normal ?
>
> Hi everybody,
>
> I try to stitch 3D stacks (5 planes, 1 channel) with the Grid/Collection
> stitching plugin but the displayed fused image is a multi-channel one with
> 1 plane, with new number of channels = number of Z-planes. Obviously
> Stitching in Z is also wrong.
> Does that mean that I can't stitch Z-stacks this way?
>
> Thanks for your help,
>
> Best regards,
>
> Laurent.
>
> ___________________________
> Laurent Gelman, PhD
> Friedrich Miescher Institut
> Facility for Advanced Imaging and Microscopy
> Head of Light Microscopy
> WRO 1066.2.16
> Maulbeerstrasse 66
> CH-4058 Basel
> +41 (0)61 696 43 38
> +41 (0)79 618 73 69
>
>
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> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
>

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