Re: Quantification of red in bright field image

Posted by Kenton Arkill on Jul 17, 2013; 12:50pm
URL: http://imagej.273.s1.nabble.com/Quantification-of-red-in-bright-field-image-tp5003997p5003998.html

Hi
These are always difficult to quantify. They are very rarely linear to colour (i.e. double brightness = double substance stained for) but if you want the area covered then:

the images are the light source colour minus the tissue absorption (assume = though colours) minus the stain. Where you see red is actually less blue and green.
If you go images/split colour you'll get the three colours separately. then Process/ image calculator and divide the red images by the blue one. You can try to then use the threshold tool to find where is red.
You'll notice that even though it does not look it your image is not evenly illuminated which makes this harder. There are some dark red blotches in the original, if you are after those it works very well and they are nicely bright.

Somebody might have more experience of this than me though.
Kenton


On 17 Jul 2013, at 11:27, Louise Grant wrote:

> Hello,
>
>
>
> I`d like to quantify the amount of red in this histo slide using ImageJ. I
> am not experience with microscopes and I´m not sure where to start. I
> searched the ImageJ archives but everything seems to be for fluorescent
> images and not brightfield.
>
>
>
> Any help would be great!  
>
>
>
> Thanks
>
>
>
> Louise
>
>
>
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html
> <0H3V 0XD 13_4_4 x20 20thJune13 LG 3 (2).jpg>

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html