Posted by
Cameron Nowell-2 on
Jul 17, 2013; 12:57pm
URL: http://imagej.273.s1.nabble.com/Quantification-of-red-in-bright-field-image-tp5003997p5003999.html
Hi Louise,
It can be done easily enough. I use the Fiji distribution of ImageJ (www.fiji.sc) so i am not sure if the functionality is available in the standard imagej package but the following worked for me
1. go to Image --> colour --> colour deconvolution
2. choose H AEC as te vector and press OK
3. you should get three images. Select the red channel one (Colour_2)
4. go to image adjust threshold, Make sure dark background is unselected. Adjust the threshold (or the MaxEntropy auto setting seemed to work ok).
5. Measure the image (Analyze --> Measure)
If you want a macro for doing this let me know
Cheers
Cam
Cameron J. Nowell
Research Facilities Manager
Monash Institute of Pharmaceutical Sciences
Monash University
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________________________________________
From: ImageJ Interest Group [
[hidden email]] on behalf of Louise Grant [
[hidden email]]
Sent: Wednesday, July 17, 2013 8:27 PM
To:
[hidden email]
Subject: Quantification of red in bright field image
Hello,
I`d like to quantify the amount of red in this histo slide using ImageJ. I
am not experience with microscopes and I´m not sure where to start. I
searched the ImageJ archives but everything seems to be for fluorescent
images and not brightfield.
Any help would be great!
Thanks
Louise
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